| 质粒名称: | pBbA5c-MevT(CO)-T1-MBIS(CO, ispA) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 35152 |
| 存储实验室: | Jay Keasling, Taek Soon Lee |
|
载体骨架基本信息 |
|
| 载体名称: | pBbA5c |
| 载体抗性: | 氯霉素 |
| 载体类型: | 细菌表达，合成生物学 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA |
| 物种来源: | S. cerevisiae (budding yeast) |
| 启动子 | LacUV5, pTrc |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoR1, BglII (未破坏) |
| 3' 克隆位点: | BamH1, Xho1(未破坏) |
| 5' 测序引物 | gatcgtttaggcacccca |
| 3' 测序引物 | ATTAATAAGATGATC |
|
文献引用方法 |
|
| 材料和方法部分: | pBbA5c-MevT(CO)-T1-MBIS(CO, ispA) was a gift from Jay Keasling & Taek Soon Lee (Addgene plasmid # 35152 ; http://n2t.net/addgene:35152 ; RRID:Addgene_35152) |
| 参考文献部分: | Identification and microbial production of a terpene-based advanced biofuel. Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, Lee TS. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. 10.1038/ncomms1494 PubMed 21952217 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
-T1-MBIS(CO, ispA)质粒图谱.png)
载体简介
载体序列
LOCUS pBbA5c-MevT(CO)-T1-MBIS(CO, ispA) 13541 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13541)
AUTHORS Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD,
Lee TS
TITLE Identification and microbial production of a terpene-based advanced
biofuel.
JOURNAL Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
PUBMED 21952217
REFERENCE 2 (bases 1 to 13541)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13541
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(49..1131)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 1093..1112
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(1132..1209)
/gene="lacI"
/label=lacI promoter
primer_bind 1415..1434
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 1520..1550
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
protein_bind 1558..1574
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 5220..5231
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
terminator 5849..5920
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 5936..5963
/label=T7Te terminator
/note="phage T7 early transcription terminator"
promoter 6175..6204
/label=trc promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 6212..6228
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 9251..9262
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
terminator 11706..11777
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 11793..11820
/label=T7Te terminator
/note="phage T7 early transcription terminator"
primer_bind complement(11848..11865)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11982..12527)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(12062..12081)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 12641..12735
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(12756..13415)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 13330..13349
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(13416..13518)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
ORIGIN
1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
1561 tgagcggata acaatttcag aattcaaaag atctaaagga ggccatcctg gccatgaaga
1621 actgtgtgat tgtttctgcg gtccgcacgg cgatcggcag ctttaacggc tctttagcga
1681 gcacctctgc aatcgatctg ggtgcgacgg tcattaaggc cgccattgaa cgcgccaaaa
1741 tcgacagcca gcacgttgat gaggtgatca tgggcaatgt gttacaagcc ggcctgggtc
1801 aaaacccagc gcgtcaagca ctgttaaaat ctggtctggc cgagaccgtg tgtggcttca
1861 ccgtcaataa ggtttgcggc tctggcctga agagcgtggc cctggcagca caagcgattc
1921 aagccggtca ggcacaaagc atcgttgcgg gtggcatgga gaacatgtct ctggcgccgt
1981 acttattaga tgccaaagcc cgcagcggtt atcgcctggg cgatggtcag gtgtacgacg
2041 tcatcttacg cgatggctta atgtgcgcga cccacggtta ccacatgggt attacggccg
2101 aaaacgtggc gaaagaatac ggcattacgc gcgagatgca ggatgaatta gcactgcact
2161 ctcagcgcaa agcagcagcc gcgatcgagt ctggtgcgtt tacggcggaa atcgtgccag
2221 ttaacgtggt cacgcgcaag aagacgttcg ttttcagcca ggacgagttc ccgaaggcaa
2281 acagcaccgc ggaggcctta ggtgccttac gcccagcctt tgacaaagcg ggcacggtca
2341 ccgccggtaa tgcgagcggc atcaatgatg gtgcagcggc actggtcatc atggaagaga
2401 gcgccgcatt agcagcgggt ctgaccccat tagcgcgcat taaatcttat gccagcggcg
2461 gcgtcccacc agccctgatg ggcatgggtc cggtcccagc cacgcaaaaa gccctgcaat
2521 tagcgggcct gcaactggcc gacattgatc tgatcgaggc gaacgaggcg tttgcagcgc
2581 agttcctggc ggtgggtaag aatctgggct tcgacagcga gaaagtcaat gtgaacggtg
2641 gcgcgattgc gttaggccat ccgattggtg caagcggcgc acgcatctta gtgacgttac
2701 tgcacgccat gcaggcacgc gacaagacct taggcctggc gaccttatgt attggtggcg
2761 gtcaaggtat cgccatggtg atcgaacgcc tgaactgatg aaggaggaaa gcaaaatgaa
2821 actgagcacc aagctgtgct ggtgtggcat caagggtcgc ctgcgcccac aaaagcagca
2881 acagctgcac aacacgaacc tgcaaatgac cgagctgaaa aagcagaaga cggccgagca
2941 aaagacccgc ccgcagaacg ttggcatcaa gggcatccag atttatatcc cgacgcagtg
3001 tgtcaaccaa tctgagctgg agaaattcga tggcgtcagc cagggtaagt acaccatcgg
3061 cctgggccag accaacatga gcttcgtgaa cgaccgtgag gacatctatt ctatgagcct
3121 gacggtgctg tctaagctga tcaagagcta caacatcgac acgaataaga tcggtcgtct
3181 ggaggtgggt acggagacgc tgattgacaa gagcaaaagc gtgaagtctg tcttaatgca
3241 gctgttcggc gagaacacgg atgtcgaggg tatcgacacc ctgaacgcgt gttacggcgg
3301 caccaacgca ctgttcaata gcctgaactg gattgagagc aacgcctggg atggccgcga
3361 tgcgatcgtc gtgtgcggcg atatcgccat ctatgacaag ggtgcggcac gtccgaccgg
3421 cggtgcaggc accgttgcga tgtggattgg cccggacgca ccaattgtct tcgattctgt
3481 ccgcgcgtct tacatggagc acgcctacga cttttacaag ccggacttca cgagcgaata
3541 cccgtacgtg gacggccact tctctctgac ctgctatgtg aaggcgctgg accaggttta
3601 taagtcttat agcaaaaagg cgatttctaa gggcctggtc agcgacccgg caggcagcga
3661 cgccctgaac gtgctgaagt atttcgacta caacgtgttc catgtcccga cctgcaaatt
3721 agtgaccaaa tcttatggcc gcctgttata taatgatttc cgtgccaacc cgcagctgtt
3781 cccggaggtt gacgccgagc tggcgacgcg tgattacgac gagagcctga ccgacaagaa
3841 catcgagaag accttcgtca acgtcgcgaa gccgttccac aaagagcgtg tggcccaaag
3901 cctgatcgtc ccgaccaaca cgggcaacat gtataccgcg tctgtctacg cggcattcgc
3961 gagcctgctg aattacgtcg gttctgacga cctgcagggc aagcgcgttg gcctgttcag
4021 ctacggtagc ggcttagcgg ccagcctgta tagctgcaaa attgtcggcg acgtccagca
4081 catcatcaag gagctggaca tcaccaacaa gctggcgaag cgcatcaccg agacgccgaa
4141 agattacgag gcagcgatcg agttacgcga gaatgcgcat ctgaagaaga acttcaagcc
4201 gcaaggtagc atcgagcacc tgcagagcgg cgtctactac ctgacgaaca ttgacgacaa
4261 gttccgccgt tcttatgacg tcaaaaagta actagtagga ggaaaacatc atggtgctga
4321 cgaacaaaac cgtcattagc ggcagcaagg tgaagtctct gagcagcgcc caaagctcta
4381 gcagcggccc gtctagcagc agcgaggagg acgacagccg tgacattgag tctctggaca
4441 agaagatccg cccgctggag gagttagagg ccctgctgag cagcggcaac accaagcagc
4501 tgaagaacaa ggaagttgca gcgctggtga tccacggtaa gctgccactg tatgcgctgg
4561 aaaagaaact gggcgatacg acgcgtgcgg tcgcggtgcg tcgcaaagcc ttaagcatct
4621 tagcggaggc cccggtgtta gccagcgacc gcctgccgta caagaactac gactacgacc
4681 gcgtgtttgg cgcgtgctgc gagaatgtca ttggctacat gccgttaccg gttggtgtga
4741 tcggcccgct ggtcattgat ggcacgagct atcacattcc aatggcgacc acggaaggtt
4801 gcttagtcgc cagcgccatg cgtggctgta aggcgattaa cgccggcggt ggcgcgacga
4861 ccgtgttaac caaggatggt atgacgcgcg gtccggtcgt ccgcttccca acgctgaagc
4921 gcagcggcgc gtgtaagatt tggctggatt ctgaggaggg ccaaaacgcg atcaagaaag
4981 ccttcaactc tacgagccgt ttcgcgcgtt tacagcatat ccagacctgc ctggccggcg
5041 acctgctgtt catgcgcttc cgcaccacca cgggcgatgc gatgggcatg aacatgatca
5101 gcaagggcgt cgaatatagc ctgaaacaaa tggtggaaga atatggctgg gaggacatgg
5161 aggttgtctc tgtgagcggc aactattgca ccgacaagaa gccggcagcc attaactgga
5221 ttgagggtcg cggcaaaagc gtcgtggcag aagcgaccat cccaggcgac gtggtccgta
5281 aggttctgaa gagcgacgtc agcgccctgg ttgagttaaa tatcgcgaaa aacctggtcg
5341 gcagcgcgat ggcgggcagc gtgggtggct ttaacgcaca tgcagcgaat ctggttacgg
5401 cggttttctt agccttaggt caggacccag cccaaaatgt cgagagcagc aactgcatta
5461 ccttaatgaa agaggttgac ggtgacctgc gcatcagcgt ttctatgccg tctatcgagg
5521 tcggcacgat cggcggcggc accgttttag aaccgcaagg tgcgatgctg gatctgctgg
5581 gcgtgcgcgg cccacatgca acggccccag gcaccaatgc ccgccaactg gcccgtatcg
5641 tggcctgcgc ggttctggcg ggtgagctga gcctgtgcgc cgcattagcc gcgggccatt
5701 tagttcaatc tcacatgacc cacaaccgca agccggcaga accaaccaag ccaaataacc
5761 tggacgcaac cgacattaac cgtctgaagg atggcagcgt cacgtgcatt aaaagctgat
5821 gaggatcttt ttaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg
5881 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
5941 accttcgggt gggcctttct gcgtttatag cgaattgatc tggtttgaca gcttatcatc
6001 gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct
6061 gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
6121 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca
6181 attaatcatc cggctcgtat aatgtgtgga attgtgagcg gataacaatt tcaggatcta
6241 ggaggaaata accatgtctc tgccattcct gacgtctgcg ccaggtaagg tgatcatctt
6301 cggcgagcac tctgcggtgt acaataagcc ggccgtcgcc gcctctgtgt ctgcgttacg
6361 cacctacctg ctgatcagcg aatcttctgc accggacacg atcgagctgg actttccgga
6421 catcagcttc aaccacaagt ggagcatcaa cgacttcaac gcgatcacgg aggaccaggt
6481 gaacagccaa aagctggcca aagcccagca agcaaccgac ggtctgtctc aggagctggt
6541 gtctctgctg gacccgctgt tagcgcagtt aagcgagagc ttccattacc acgccgcgtt
6601 ctgcttcctg tacatgttcg tttgcctgtg cccgcacgca aagaacatca agttcagcct
6661 gaagagcacg ctgccgattg gcgcaggctt aggctctagc gcatctatca gcgtgagcct
6721 ggcgctggcg atggcctatc tgggtggcct gattggcagc aacgacctgg agaaactgag
6781 cgaaaacgac aagcacatcg tgaaccagtg ggcctttatc ggcgagaagt gcattcatgg
6841 caccccgagc ggcattgaca acgcagttgc cacgtatggc aacgccctgc tgttcgagaa
6901 agacagccac aacggcacga tcaacacgaa caacttcaag ttcctggacg acttcccggc
6961 gatcccgatg attctgacct acacccgtat cccacgcagc accaaggatt tagtcgcccg
7021 cgtgcgtgtt ttagtcaccg aaaagttccc ggaggtgatg aagccgatcc tggacgcgat
7081 gggcgagtgc gcgctgcagg gtctggagat catgaccaag ctgagcaagt gcaagggcac
7141 cgacgatgag gcggtggaga ccaacaatga gctgtacgag cagctgctgg agctgatccg
7201 tatcaatcac ggcctgctgg tctctatcgg tgtgtctcac ccgggcctgg aactgatcaa
7261 aaacctgagc gacgacctgc gcattggctc tacgaaatta acgggtgcag gtggcggtgg
7321 ctgctcttta acgctgctgc gccgtgacat tacgcaggag caaatcgaca gcttcaagaa
7381 gaagctgcag gacgacttca gctacgagac gttcgagacg gacctgggcg gcacgggctg
7441 ttgcctgctg agcgccaaaa atctgaacaa ggacctgaag atcaaaagcc tggtgttcca
7501 gctgttcgaa aacaagacga ccacgaagca gcagatcgac gacctgttac tgccgggtaa
7561 caccaatctg ccgtggacgt cttaaggatc taggagggag atcatatgag cgaattacgt
7621 gcattcagcg cgccaggtaa ggcactgctg gccggtggct acctggtgtt agacaccaag
7681 tacgaggcgt tcgtcgtcgg cttatctgcc cgtatgcatg cagttgccca cccgtatggt
7741 agcctgcagg gctctgacaa gttcgaagtg cgtgtgaaga gcaagcagtt caaggacggc
7801 gagtggctgt accacattag cccaaagagc ggcttcatcc cggttagcat tggtggcagc
7861 aagaacccat ttatcgagaa ggtcattgcc aacgtcttca gctacttcaa gccgaatatg
7921 gacgattact gcaaccgcaa cctgttcgtc atcgacattt tcagcgacga cgcgtaccac
7981 agccaagagg actctgttac ggagcatcgt ggtaaccgcc gcctgagctt ccacagccat
8041 cgcattgagg aggtgccgaa gacgggtctg ggttctagcg ccggtttagt taccgtctta
8101 acgacggcgt tagcgagctt cttcgtgagc gacctggaga acaacgtgga caagtaccgc
8161 gaagtgattc ataacctggc gcaggtggca cattgtcagg cccaaggtaa gattggctct
8221 ggttttgatg tggcagcggc cgcctatggc tctatccgct atcgccgctt tccgccggcc
8281 ctgatcagca atctgccgga catcggctct gcgacgtatg gtagcaaact ggcgcatctg
8341 gtggacgagg aggactggaa catcaccatt aagtctaatc acctgccgag cggcttaacg
8401 ttatggatgg gcgatatcaa gaacggcagc gaaacggtta agctggtgca gaaagtgaaa
8461 aactggtacg acagccacat gccggaaagc ctgaagattt acacggagct ggaccacgcc
8521 aatagccgtt tcatggatgg tctgagcaag ctggaccgcc tgcacgaaac ccacgacgac
8581 tacagcgacc aaatcttcga gagcctggag cgcaatgact gcacctgcca gaagtacccg
8641 gagatcacgg aggtccgcga tgccgtggca acgattcgcc gtagcttccg caaaattacg
8701 aaggagagcg gcgcggatat cgaaccaccg gtccagacgt ctctgctgga cgactgtcaa
8761 accttaaagg gcgtgttaac gtgcctgatt ccgggcgcgg gtggttacga cgccattgcc
8821 gtcatcacga aacaggacgt cgatctgcgc gcacaaacgg ccaacgacaa acgtttcagc
8881 aaagtccaat ggctggatgt tacgcaggcc gactggggtg ttcgcaagga gaaggacccg
8941 gaaacgtatc tggataagtg aggatctagg aggattatga gatgaccgtt tacacagcat
9001 ccgttaccgc acccgtcaac atcgcaaccc ttaagtattg ggggaaaagg gacacgaagt
9061 tgaatctgcc caccaattcg tccatatcag tgactttatc gcaagatgac ctcagaacgt
9121 tgacctctgc ggctactgca cctgagtttg aacgcgacac tttgtggtta aatggagaac
9181 cacacagcat cgacaatgaa agaactcaaa attgtctgcg cgacctacgc caattaagaa
9241 aggaaatgga atcgaaggac gcctcattgc ccacattatc tcaatggaaa ctccacattg
9301 tctccgaaaa taactttcct acagcagctg gtttagcttc ctccgctgct ggctttgctg
9361 cattggtctc tgcaattgct aagttatacc aattaccaca gtcaacttca gaaatatcta
9421 gaatagcaag aaaggggtct ggttcagctt gtagatcgtt gtttggcgga tacgtggcct
9481 gggaaatggg aaaagctgaa gatggtcatg attccatggc agtacaaatc gcagacagct
9541 ctgactggcc tcagatgaaa gcttgtgtcc tagttgtcag cgatattaaa aaggatgtga
9601 gttccactca gggtatgcaa ttgaccgtgg caacctccga actatttaaa gaaagaattg
9661 aacatgtcgt accaaagaga tttgaagtca tgcgtaaagc cattgttgaa aaagatttcg
9721 ccacctttgc aaaggaaaca atgatggatt ccaactcttt ccatgccaca tgtttggact
9781 ctttccctcc aatattctac atgaatgaca cttccaagcg tatcatcagt tggtgccaca
9841 ccattaatca gttttacgga gaaacaatcg ttgcatacac gtttgatgca ggtccaaatg
9901 ctgtgttgta ctacttagct gaaaatgagt cgaaactctt tgcatttatc tataaattgt
9961 ttggctctgt tcctggatgg gacaagaaat ttactactga gcagcttgag gctttcaacc
10021 atcaatttga atcatctaac tttactgcac gtgaattgga tcttgagttg caaaaggatg
10081 ttgccagagt gattttaact caagtcggtt caggcccaca agaaacaaac gaatctttga
10141 ttgacgcaaa gactggtcta ccaaaggaat aaggatctag gaggtaatga taatgcaaac
10201 ggaacacgtc attttattga atgcacaggg agttcccacg ggtacgctgg aaaagtatgc
10261 cgcacacacg gcagacaccc gcttacatct cgcgttctcc agttggctgt ttaatgccaa
10321 aggacaatta ttagttaccc gccgcgcact gagcaaaaaa gcatggcctg gcgtgtggac
10381 taactcggtt tgtgggcacc cacaactggg agaaagcaac gaagacgcag tgatccgccg
10441 ttgccgttat gagcttggcg tggaaattac gcctcctgaa tctatctatc ctgactttcg
10501 ctaccgcgcc accgatccga gtggcattgt ggaaaatgaa gtgtgtccgg tatttgccgc
10561 acgcaccact agtgcgttac agatcaatga tgatgaagtg atggattatc aatggtgtga
10621 tttagcagat gtattacacg gtattgatgc cacgccgtgg gcgttcagtc cgtggatggt
10681 gatgcaggcg acaaatcgcg aagccagaaa acgattatct gcatttaccc agcttaaata
10741 aggatctagg aggataaaga aatggacttt ccgcagcaac tcgaagcctg cgttaagcag
10801 gccaaccagg cgctgagccg ttttatcgcc ccactgccct ttcagaacac tcccgtggtc
10861 gaaaccatgc agtatggcgc attattaggt ggtaagcgcc tgcgaccttt cctggtttat
10921 gccaccggtc atatgttcgg cgttagcaca aacacgctgg acgcacccgc tgccgccgtt
10981 gagtgtatcc acgcttactc attaattcat gatgatttac cggcaatgga tgatgacgat
11041 ctgcgtcgcg gtttgccaac ctgccatgtg aagtttggcg aagcaaacgc gattctcgct
11101 ggcgacgctt tacaaacgct ggcgttctcg attttaagcg atgccgatat gccggaagtg
11161 tcggaccgcg acagaatttc gatgatttct gaactggcga gcgccagtgg tattgccgga
11221 atgtgcggtg gtcaggcatt agatttagac gcggaaggca aacacgtacc tctggacgcg
11281 cttgagcgta ttcatcgtca taaaaccggc gcattgattc gcgccgccgt tcgccttggt
11341 gcattaagcg ccggagataa aggacgtcgt gctctgccgg tactcgacaa gtatgcagag
11401 agcatcggcc ttgccttcca ggttcaggat gacatcctgg atgtggtggg agatactgca
11461 acgttgggaa aacgccaggg tgccgaccag caacttggta aaagtaccta ccctgcactt
11521 ctgggtcttg agcaagcccg gaagaaagcc cgggatctga tcgacgatgc ccgtcagtcg
11581 ctgaaacaac tggctgaaca gtcactcgat acctcggcac tggaagcgct agcggactac
11641 atcatccagc gtaataaata aggatcctaa tgaggatcca aactcgagta aggatctcca
11701 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
11761 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
11821 tttataccta gggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
11881 actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa
11941 gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc
12001 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
12061 taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
12121 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
12181 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
12241 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
12301 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
12361 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
12421 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
12481 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
12541 ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg
12601 aagtcagccc catacgatat aagttgttac tagtgcttgg attctcacca ataaaaaacg
12661 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
12721 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
12781 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
12841 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
12901 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
12961 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
13021 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
13081 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
13141 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
13201 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
13261 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
13321 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
13381 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
13441 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
13501 cctcttacgt gccgatcaac gtctcatttt cgccagatat c
//
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